Linking oral microbial proteolysis to aMMP‐8 PoC diagnostics along with the stage and grade of periodontitis: A cross‐sectional study

Timo Sorsa, Shipra Gupta, Vaibhav Sahni, Ismo T. Räisänen, Andreas Grigoriadis, Dimitra Sakellari, Dirk‐Rolf Gieselmann
2021-08-17
SCID:  54.1/28za8wq6 Discuss with AI
Treponema denticola dentilisinactive MMP-8 (aMMP-8)periodontitis staging and grading (stage, grade)point-of-care (PoC) lateral flow aMMP-8 test (PerioSafe®/ORALyzer®)prediabetes (HbA1c ≥5.7%)
Abstract (AI)
quantitative, specific, and sensitive point-of-care/chair-side oral fluid immunotests for aMMP-8 to detect periodontal and periimplant diseases.
Key Findings
1
Active MMP-8 (aMMP-8) levels measured by a PoC lateral flow mouthrinse test increase with periodontitis stage, being lowest in healthy/stage I and highest in stages II–III.
2
Patients with rapid periodontitis progression (grade C) and prediabetes (HbA1c ≥5.7%) exhibit the highest aMMP-8 levels, linking dysbiotic bacteria, prediabetes, and increased disease activity.
3
The aMMP-8 PoC test specifically detects the active/fragmented MMP-8 species generated by microbial activation and correlates with standard laboratory methods, supporting its diagnostic utility.
4
Total/latent salivary MMP-8 levels do not show statistically significant differences across healthy and periodontitis patients (p = 0.180), unlike aMMP-8.
5
Treponema denticola dentilisin protease converts 75 kDa latent proMMP-8 into 65 kDa active and lower molecular weight fragmented MMP-8 species in a time-dependent manner (shown by Western blot).
Research Object

Active matrix metalloproteinase-8 (aMMP-8) levels measured by point-of-care (PoC) oral fluid tests in patients with varying stages and grades of periodontitis and associated oral dysbiotic bacteria (notably Treponema denticola and its dentilisin protease)

Research Subject

Relationship between microbial proteolysis (especially T. denticola dentilisin activity), prediabetes status, and activated aMMP-8 detection by PoC diagnostics as a marker of periodontitis disease activity, stage (severity) and grade (rate of progression)

Publication Details
Publication Date
2021-08-17
Journal
Publisher
ISSN
Cited by
31
Access Type
Author Information
Authors
Timo Sorsa
Shipra Gupta
Vaibhav Sahni
Ismo T. Räisänen
Andreas Grigoriadis
Dimitra Sakellari
Dirk‐Rolf Gieselmann
Explore further
Open the scid.ai AI chat with a ready-made request: it will find papers on a similar topic and help build a literature review.
Find similar papers in the chat →
Make a presentation
100%