Hypsizygus marmoreus as a Source of Indole Compounds and Other Bioactive Substances with Health-Promoting Activities
2022-12-15
SCID: 54.1/4vrfh7sa
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5-hydroxy-L-tryptophan5-methyltryptamineDPPH FRAP ABTS antioxidant assaysHypsizygus marmoreusL-tryptophanZn and Mg enrichmentbioelements (Na, Ca, Zn, Mg)ergothioneineflame atomic absorption spectrometryglucanshigh-performance liquid chromatographyin vitro mycelial culturesindole compoundslovastatinmelatoninprotocatechuic acidsterolstryptamine
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Abstract (AI)
<i>Hypsizygus marmoreus</i> is an edible medicinal mushroom species with a high dietary value. In this study, the fruiting bodies of commercial and self-cultivated crops and mycelium from in vitro <i>H. marmoreus</i> cultures (both white and brown varieties) were evaluated. This study aimed to analyze the presence of indole compounds and other biologically active substances and determine the effect that the addition of zinc and magnesium ions to the culture medium has on the content of the tested compounds in mycelial cultures. The content of indole compounds and other organic compounds was determined using high-performance liquid chromatography, the content of bioelements was determined using flame atomic absorption spectrometry, the glucan content was determined spectrophotometrically, and the antioxidant activity of extracts was estimated using DPPH, FRAP, and ABTS methods. The results showed that <i>H. marmoreus</i> mycelium from in vitro cultures is a good source of indole compounds, bioelements, glucans, and lovastatin. Mycelia from in vitro cultures showed the most diverse composition of indole compounds (L-tryptophan, 5-hydroxy-L-tryptophan, tryptamine, 5-methyltryptamine, and melatonin). Additionally, in vitro cultures of <i>H. marmoreus</i> enriched with Zn and Mg salts increased the contents of Na, Ca, Zn, 5-methyltryptamine, melatonin, protocatechuic acid, sterols, and total glucans. Only in the case of the white variety of mycelial enriched cultures, ergothioneine and Mg levels also increased. To summarize, the content of the active compounds differed depending on the <i>H. marmoreus</i> variety and the tested material.
Key Findings
1
Enriching in vitro H. marmoreus cultures with Zn and Mg salts increased contents of Na, Ca, Zn, 5-methyltryptamine, melatonin, protocatechuic acid, sterols, and total glucans.
2
Hypsizygus marmoreus mycelium from in vitro cultures is a good source of indole compounds, bioelements, glucans, and lovastatin.
3
In the white variety of enriched mycelial cultures, ergothioneine and Mg levels additionally increased.
4
Mycelia from in vitro cultures exhibited the most diverse indole composition: L-tryptophan, 5-hydroxy-L-tryptophan, tryptamine, 5-methyltryptamine, and melatonin.
5
The content of active compounds varied depending on H. marmoreus variety (white vs brown) and the type of tested material (fruiting bodies vs mycelium).
Research Object
Hypsizygus marmoreus fruiting bodies and in vitro mycelial cultures (white and brown varieties)
Research Subject
Presence and content of indole compounds and other bioactive substances (bioelements, glucans, lovastatin, sterols, ergothioneine) and effects of Zn and Mg enrichment on these contents and antioxidant activity
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2022-12-15
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