Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation

Иммобилизированный Stenotrophomonas maltophilia KB2 при деградации напроксена
Danuta Wojcieszyńska, Judyta Klamka, Ariel Marchlewicz, Izabela Potocka, Joanna Żur, Urszula Guzik
2022-09-07

Luffa cylindrica spongeStenotrophomonas maltophilia KB2cell immobilizationcometabolic bioremediationnaproxen biodegradation
Immobilization is a commonly used method in response to the need to increase the resistance of microorganisms to the toxic effects of xenobiotics. In this study, a plant sponge from Luffa cylindrica was used as a carrier for the immobilization of the Stenotrophomonas maltophilia KB2 strain since such a carrier meets the criteria for high-quality carriers, i.e., low price and biodegradability. The optimal immobilization conditions were established as a temperature of 30 °C, pH 7.2, incubation time of 72 h, and an optical density of the culture of 1.4. The strain immobilized in such conditions was used for the biodegradation of naproxen, and an average rate of degradation of 3.8 µg/hour was obtained under cometabolic conditions with glucose. The obtained results indicate that a microbiological preparation based on immobilized cells on a luffa sponge can be used in bioremediation processes where it is necessary to remove the introduced carrier.
1
Immobilized S. maltophilia KB2 degraded naproxen under glucose-assisted cometabolic conditions at an average rate of 3.8 µg/hour.
2
Luffa cylindrica plant sponge was successfully used as a low-cost, biodegradable carrier for immobilizing Stenotrophomonas maltophilia KB2.
3
Optimal immobilization conditions were 30 °C, pH 7.2, 72 hours of incubation, and culture optical density 1.4.
4
The immobilized-cell preparation may support bioremediation applications requiring subsequent removal of the carrier material.

Luffa cylindrica sponge-immobilized Stenotrophomonas maltophilia KB2 cells applied to naproxen-containing systems

Naproxen biodegradation performance under optimized immobilization and glucose-cometabolic conditions

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2022-09-07
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Authors
Danuta Wojcieszyńska
Judyta Klamka
Ariel Marchlewicz
Izabela Potocka
Joanna Żur
Urszula Guzik
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