Acetaldehyde production by Rothia mucilaginosa isolates from patients with oral leukoplakia

Выработка ацетальдегида изолятами Rothia mucilaginosa у пациентов с оральной лейкоплакией
Claire M. Healy, Abdrazak Amer, Áine M. Whelan, Nezar N. Al‐Hebshi, Gary P. Moran
2020-01-01

Acetaldehyde productionRothia mucilaginosaoral leukoplakiapotentially malignant disorders
Rothia mucilaginosa has been found at high abundance on oral leukoplakia (OLK). The ability of clinical isolates to produce acetaldehyde (ACH) from ethanol has not been investigated. The objective of the current study was to determine the capacity of R. mucilaginosa isolates recovered from OLK to generate ACH. Analysis of R. mucilaginosa genomes (n = 70) shows that this species does not normally encode acetaldehyde dehydrogenase (ALDH) required for detoxification of ACH. The predicted OLK metagenome also exhibited reduced ALDH coding capacity. We analysed ACH production in 8 isolates of R. mucilaginosa and showed that this species is capable of generating ACH in the presence of ethanol. The levels of ACH produced (mean = 53 µM) were comparable to those produced by Neisseria mucosa and Candida albicans in parallel assays. These levels were demonstrated to induce oxidative stress in cultured oral keratinocytes. This study shows that R. mucilaginosa can generate ACH from ethanol in vitro at levels which can induce oxidative stress. This organism likely contributes to oral ACH levels following alcohol consumption and the significance of the increased abundance of R. mucilaginosa in patients with potentially malignant disorders requires further investigation.
1
Acetaldehyde production by these isolates suggests a possible microbial contribution to oral potentially malignant disorders
2
Isolates originated from patients with oral leukoplakia, linking R. mucilaginosa to acetaldehyde presence in that condition
3
Rothia mucilaginosa isolates studied were identified as producers of acetaldehyde

Rothia mucilaginosa isolates from patients with oral leukoplakia

Production of acetaldehyde by these Rothia mucilaginosa isolates

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2020-01-01
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Authors
Claire M. Healy
Abdrazak Amer
Áine M. Whelan
Nezar N. Al‐Hebshi
Gary P. Moran
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