Recombinant protein expression in Escherichia coli: advances and challenges
Экспрессия рекомбинантных белков в Escherichia coli: достижения и проблемы
2014-04-17
SCID: 54.1/68xpyb4n
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Escherichia coliengineered strainsexpression plasmidsheterologous protein productionrecombinant protein expression
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Abstract (AI)
Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
Key Findings
1
Engineered E. coli strains and diverse cultivation strategies expand the available approaches for recombinant protein synthesis.
2
Escherichia coli remains one of the leading and most widely used cell factories for recombinant protein production.
3
High-level heterologous protein expression in E. coli is supported by extensive molecular tools, including numerous expression plasmids.
4
The field has achieved substantial recent progress but continues to face challenges requiring ongoing methodological development.
Research Object
Recombinant protein production in Escherichia coli
Research Subject
Approaches, advances, and challenges in the high-level synthesis of heterologous proteins, including expression tools, engineered strains, and cultivation strategies
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2014-04-17
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