Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius
Сборка генома и геопространственная филогеномика постельного клопа Cimex lectularius
2016-02-02
SCID: 54.1/7tz9s4uw
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Cimex lectularius genomeRNA-seqWolbachia endosymbiontgenome assemblygeospatial phylogenomics
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Abstract (AI)
The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971 kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding and noncoding gene models. The most pronounced change in gene expression during the life cycle occurs after feeding on human blood and included genes from the Wolbachia endosymbiont, which shows a simultaneous and coordinated host/commensal response to haematophagous activity. These data provide a rich genetic resource for mapping activity and density of C. lectularius across human hosts and cities, which can help track, manage and control bed bug infestations.
Key Findings
1
A 697.9-Mb Cimex lectularius genome was assembled and annotated using combined long- and short-read sequencing, achieving a 971-kb N50.
2
Blood feeding triggered coordinated transcriptional responses in both bed bugs and their Wolbachia endosymbiont.
3
RNA-seq across five developmental stages and both adult sexes identified 36,985 coding and noncoding gene models.
4
The genome and expression resources enable phylogenetic and geospatial studies to track, manage, and control bed bug infestations across hosts and cities.
5
The strongest life-cycle gene-expression shift occurred after feeding on human blood.
Research Object
the common bed bug (Cimex lectularius), including its genome, developmental stages, and Wolbachia endosymbiont
Research Subject
the genetic basis of bed bug biology and adaptation to dense human environments, including life-cycle gene expression and coordinated host–Wolbachia responses to human-blood feeding
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2016-02-02
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