The effect of melatonin on in vitro fertilization and embryo development in mice

Влияние мелатонина на оплодотворение in vitro и развитие эмбрионов у мышей
Yasushi Kuribayashi, Akira Amemiya, Bunpei Ishizuka, Kunihiko Murai, Masanori Itoh
2000-01-01
SCID:  54.1/83yphhxc Discuss with AI
blastulationembryonic developmentin vitro fertilizationmelatoninmouse embryos
Abstract (AI)
To examine the effect of melatonin on in vitro fertilization and embryonic development, mouse embryos after insemination in vitro were cultured in a physiological medium with or without melatonin. Melatonin increased the fertilization rate significantly at a concentration between 10(-6) and 10(-4) M (27.6 vs. 43.9 or 40.4%, P < 0.01). Furthermore, a significant increase in the rate of embryos reaching the four-cell stage (16.0 vs. 26.7%, P < 0.01), the eight-cell stage (12.1 vs. 25.8 or 23.5%, P < 0.01), and blastulation (8.9 vs. 23.5 or 17.5%, P < 0.01) was observed when the embryos were cultured in a medium containing 10(-8) or 10(-6) M melatonin. These results demonstrate that melatonin supports fertilization and early embryo development after in vitro fertilization.
Key Findings
1
Melatonin at 10^-8 or 10^-6 M significantly increased blastulation rates, from 8.9% to 23.5% or 17.5%.
2
Melatonin at 10^-8 or 10^-6 M significantly increased eight-cell-stage development, from 12.1% to 25.8% or 23.5%.
3
Melatonin at 10^-8 or 10^-6 M significantly increased the proportion of embryos reaching the four-cell stage, from 16.0% to 26.7%.
4
Melatonin significantly increased mouse in vitro fertilization rates at concentrations of 10^-6 to 10^-4 M, from 27.6% to 43.9% or 40.4%.
5
The findings indicate that melatonin supports both fertilization and early embryonic development after in vitro fertilization in mice.
Research Object

Mouse in vitro fertilization and early embryos cultured in physiological medium with melatonin

Research Subject

the effects of melatonin concentration on fertilization success and early embryonic development, including progression to the four-cell, eight-cell, and blastulation stages

Publication Details
Publication Date
2000-01-01
Journal
Publisher
ISSN
Access Type
Author Information
Authors
Yasushi Kuribayashi
Akira Amemiya
Bunpei Ishizuka
Kunihiko Murai
Masanori Itoh
Explore further
Open the scid.ai AI chat with a ready-made request: it will find papers on a similar topic and help build a literature review.
Find similar papers in the chat →
Make a presentation
100%