A Direct Electron Transfer‐Based Glucose/Oxygen Biofuel Cell Operating in Human Serum

Биотопливный элемент на основе прямого переноса электронов, работающий на глюкозе и кислороде в сыворотке крови человека
Vasile Coman, Roland Ludwig, Wolfgang Harreither, Dietmar Haltrich, Lo Gorton, Tautgirdas Ruzgas, Sergey Shleev
2009-10-07

bilirubin oxidasecellobiose dehydrogenasedirect electron transferglucose/oxygen biofuel cellhuman serum
Abstract We report on the fabrication and characterisation of the very first direct electron transfer‐based glucose/oxygen biofuel cell (BFC) operating in neutral glucose‐containing buffer and human serum. Corynascus thermophilus cellobiose dehydrogenase and Myrothecium verrucaria bilirubin oxidase were used as anodic and cathodic bioelements, respectively. The following characteristics of the mediator‐, separator‐ and membrane‐less, a priori, non‐toxic and simple miniature BFC, was obtained: an open‐circuit voltage of 0.62 and 0.58 V, a maximum power density of ca. 3 and 4 μW cm–2 at 0.37 and 0.19 V of cell voltage, in phosphate buffer and human serum, respectively.
1
Corynascus thermophilus cellobiose dehydrogenase served as the anode bioelement, while Myrothecium verrucaria bilirubin oxidase served as the cathode bioelement.
2
In human serum, the cell achieved a 0.58 V open-circuit voltage and approximately 4 μW cm−2 maximum power density at 0.19 V.
3
In phosphate buffer, the cell achieved a 0.62 V open-circuit voltage and approximately 3 μW cm−2 maximum power density at 0.37 V.
4
The miniature biofuel cell was designed without mediators, separators, or membranes and was described as a priori non-toxic and simple.
5
The study reports the first direct electron transfer-based glucose/oxygen biofuel cell demonstrated in both neutral glucose buffer and human serum.

A direct electron transfer-based glucose/oxygen biofuel cell operating in human serum

The biofuel cell’s electrochemical performance and operation in neutral glucose-containing buffer and human serum, including open-circuit voltage and maximum power density

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2009-10-07
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Authors
Vasile Coman
Roland Ludwig
Wolfgang Harreither
Dietmar Haltrich
Lo Gorton
Tautgirdas Ruzgas
Sergey Shleev
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