A DNA barcode for land plants

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CBOL Plant Working Group1, Peter M. Hollingsworth, Laura L. Forrest, John L. Spouge, Mehrdad Hajibabaei, Sujeevan Ratnasingham, Michelle van der Bank, Mark W. Chase, Robyn S. Cowan, David L. Erickson, Aron J. Fazekas, Sean W. Graham, Karen E. James, Ki-Joong Kim, W. John Kress, Harald Schneider, Jonathan Van Alphen-Stahl, Spencer C. H. Barrett, Cássio van den Berg, Diego Bogarín, Kevin S. Burgess, Kenneth M. Cameron, Mark A. Carine, Juliana Chacón, Alexandra Clark, James J. Clarkson, Ferozah Conrad, Dion S. Devey, C. S. Ford, Terry A. Hedderson, Michelle L. Hollingsworth, Brian C. Husband, Laura J. Kelly, Prasad Kesanakurti, Jung Sung Kim, Young‐Dong Kim, Renaud Lahaye, Hae-Lim Lee, David G. Long, Santiago Madriñán, Olivier Maurin, Isabelle Meusnier, Steven G. Newmaster, Chong-Wook Park, Diana M. Percy, Gitte Petersen, James Richardson, Gerardo A. Salazar, Vincent Savolainen, Ole Seberg, M. J. Wilkinson, Dong‐Keun Yi, Damon P. Little
2009-08-04
SCID:  54.1/aay2b62q Discuss with AI
DNA barcodingland plantsplastid DNA regionsrbcL+matK barcodespecies identification
Abstract (AI)
DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF-atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK-psbI spacer, and trnH-psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.
Key Findings
1
Candidate regions were evaluated by recoverability, sequence quality, and species-discrimination performance.
2
The authors recommend the two-locus combination of rbcL and matK as the standard core barcode for land plants.
3
The proposed barcode provides a universal framework for routine specimen identification and may facilitate discovery of overlooked plant species.
4
The study compares seven leading plastid DNA regions for their suitability as standardized barcodes for land-plant species identification.
Research Object

land plants and their candidate plastid DNA barcode regions

Research Subject

the recoverability, sequence quality, and species-discrimination performance of candidate plastid DNA regions for plant identification

Publication Details
Publication Date
2009-08-04
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Authors
CBOL Plant Working Group1
Peter M. Hollingsworth
Laura L. Forrest
John L. Spouge
Mehrdad Hajibabaei
Sujeevan Ratnasingham
Michelle van der Bank
Mark W. Chase
Robyn S. Cowan
David L. Erickson
Aron J. Fazekas
Sean W. Graham
Karen E. James
Ki-Joong Kim
W. John Kress
Harald Schneider
Jonathan Van Alphen-Stahl
Spencer C. H. Barrett
Cássio van den Berg
Diego Bogarín
Kevin S. Burgess
Kenneth M. Cameron
Mark A. Carine
Juliana Chacón
Alexandra Clark
James J. Clarkson
Ferozah Conrad
Dion S. Devey
C. S. Ford
Terry A. Hedderson
Michelle L. Hollingsworth
Brian C. Husband
Laura J. Kelly
Prasad Kesanakurti
Jung Sung Kim
Young‐Dong Kim
Renaud Lahaye
Hae-Lim Lee
David G. Long
Santiago Madriñán
Olivier Maurin
Isabelle Meusnier
Steven G. Newmaster
Chong-Wook Park
Diana M. Percy
Gitte Petersen
James Richardson
Gerardo A. Salazar
Vincent Savolainen
Ole Seberg
M. J. Wilkinson
Dong‐Keun Yi
Damon P. Little
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