Comparative Genomics of Vibrio cholerae from Haiti, Asia, and Africa

Сравнительная геномика Vibrio cholerae из Гаити, Азии и Африки
Aaron Petkau, Gary Van Domselaar, Lori A. Rowe, Jacques Boncy, Matthew W. Gilmour, Matthew G. Walker, Morag Graham, Steven Stroika, Cheryl L. Tarr, Aleisha Reimer, Heather Kent, Deborah F. Talkington, Melissa Olsen-Rasmussen, Michael Frace, Scott Sammons, Georges Dahourou, Anthony M. Smith, Philip Mabon, Peter Gerner‐Smidt
2011-11-01
SCID:  54.1/c8sqzdtb Discuss with AI
Comparative genomicsCore genome SNP phylogenyPulsed-field gel electrophoresisVibrio choleraeWhole-genome sequencing
Abstract (AI)
Cholera was absent from the island of Hispaniola at least a century before an outbreak that began in Haiti in the fall of 2010. Pulsed-field gel electrophoresis (PFGE) analysis of clinical isolates from the Haiti outbreak and recent global travelers returning to the United States showed indistinguishable PFGE fingerprints. To better explore the genetic ancestry of the Haiti outbreak strain, we acquired 23 whole-genome Vibrio cholerae sequences: 9 isolates obtained in Haiti or the Dominican Republic, 12 PFGE pattern-matched isolates linked to Asia or Africa, and 2 nonmatched outliers from the Western Hemisphere. Phylogenies for whole-genome sequences and core genome single-nucleotide polymorphisms showed that the Haiti outbreak strain is genetically related to strains originating in India and Cameroon. However, because no identical genetic match was found among sequenced contemporary isolates, a definitive genetic origin for the outbreak in Haiti remains speculative.
Key Findings
1
No identical match was identified among contemporary sequenced isolates, so the definitive genetic origin of the Haiti outbreak remains unresolved.
2
PFGE analysis found indistinguishable fingerprints between Haiti outbreak isolates and recent global travelers’ Vibrio cholerae isolates returning to the United States.
3
Whole-genome phylogenies and core-genome single-nucleotide polymorphisms linked the Haiti outbreak strain genetically to strains from India and Cameroon.
4
Whole-genome sequencing analyzed 23 V. cholerae isolates from Haiti, the Dominican Republic, Asia, Africa, and Western Hemisphere outliers.
Research Object

Vibrio cholerae isolates from the 2010 Haiti outbreak and related isolates from Asia, Africa, and the Dominican Republic

Research Subject

Comparative whole-genome phylogenetic relationships and genetic ancestry of the Haiti outbreak strain

Publication Details
Publication Date
2011-11-01
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Authors
Aaron Petkau
Gary Van Domselaar
Lori A. Rowe
Jacques Boncy
Matthew W. Gilmour
Matthew G. Walker
Morag Graham
Steven Stroika
Cheryl L. Tarr
Aleisha Reimer
Heather Kent
Deborah F. Talkington
Melissa Olsen-Rasmussen
Michael Frace
Scott Sammons
Georges Dahourou
Anthony M. Smith
Philip Mabon
Peter Gerner‐Smidt
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