Selection of Bacterial Virulence Genes That Are Specifically Induced in Host Tissues
Выделение генов вирулентности бактерий, специально индуцируемых в тканях хозяина
1993-01-29
SCID: 54.1/dehmnhtm
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Salmonella typhimuriumhost-specific gene inductionin vivo-induced (ivi) genespositive selection genetic systemvirulence defects
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Abstract (AI)
A genetic system was devised that positively selects for bacterial genes that are specifically induced when bacteria infect their host. With the pathogen Salmonella typhimurium, the genes identified by this selection show a marked induction in bacteria recovered from mouse spleen. Mutations in all ivi (in vivo-induced) genes that were tested conferred a defect in virulence. This genetic system was designed to be of general use in a wide variety of bacterial-host systems and has several applications in both vaccine and antimicrobial drug development.
Key Findings
1
A genetic positive-selection system was developed to identify bacterial genes specifically induced during host infection.
2
Mutations in all tested in vivo-induced (ivi) genes caused defects in bacterial virulence.
3
The genetic system is designed for general use across diverse bacterial-host systems and can aid vaccine and antimicrobial drug development.
4
Using Salmonella typhimurium, genes identified by this system showed marked induction in bacteria recovered from mouse spleen.
Research Object
Bacterial virulence genes specifically induced in host tissues (in vivo-induced, ivi genes) in Salmonella typhimurium
Research Subject
Selection and identification of genes that are specifically induced during host infection and their role in virulence (induction in bacteria recovered from mouse spleen and virulence defects of ivi mutants)
Publication Details
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1993-01-29
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