ASSAY OF MALT β-GLUCANASE USING AZO-BARLEY GLUCAN: AN IMPROVED PRECIPITANT
ОПРЕДЕЛЕНИЕ АКТИВНОСТИ β-ГЛЮКАНАЗЫ СОЛОДА С ИСПОЛЬЗОВАНИЕМ АЗО-БАРЛЕЙНОГО ГЛЮКАНА: УЛУЧШЕННЫЙ ОСАДИТЕЛЬ
1987-03-04
SCID: 54.1/f7yp3mrg
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azo-barley glucancellulase activitylichenase activitymalt β-glucanase assayzinc acetate precipitant
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Abstract (AI)
A procedure recently described for the assay of malt β-glucanase, which employs a dye-labelled and chemically-modified barley β-glucan substrate, has been improved by changing the precipitant solution used to terminate the reaction. The new precipitant solution contains 0·4% (w/v) zinc acetate and 4% (w/v) sodium acetate dissolved in 80% (v/v) aqueous methyl cellosolve. With this precipitant the procedure can be directly applied to the assay of cellulase activity, and with minor modification, to the assay of lichenase activity.
Key Findings
1
The improved precipitant contains 0.4% (w/v) zinc acetate and 4% (w/v) sodium acetate in 80% (v/v) aqueous methyl cellosolve.
2
The malt β-glucanase assay using dye-labelled, chemically modified barley β-glucan was improved by changing the reaction-terminating precipitant.
3
The revised procedure can be directly applied to cellulase activity assays.
4
With minor modification, the method can also assay lichenase activity.
Research Object
malt β-glucanase acting on dye-labelled, chemically modified barley β-glucan
Research Subject
Improved enzyme assay performance using a zinc acetate–sodium acetate precipitant, including applicability to cellulase and lichenase activity assays
Publication Details
Publication Date
1987-03-04
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