Preparation of iron oxide nanoparticles functionalized with Y-shaped ligands for brain tumor targeting
Получение наночастиц оксида железа, функционализированных Y-образными лигандами, для нацеливания на опухоли головного мозга
2016-01-01
SCID: 54.1/f9jswxzr
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Y-shaped ligandschlorin e6glioblastoma targetingiron oxide nanoparticlesphotodynamic therapy
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Abstract (AI)
) particles were stabilized with pluronic F127 and coupled with dopamine-terminated Y-shaped ligands (Tat peptide and transferrin) as a result of the noncovalent conjugation of dopamine (of Y-shaped ligands) and the iron oxide nanoparticles. Here, the hydrophobic domain of pluronic F127 coated on the iron oxide nanoparticles enabled the absorption of a model photosensitizing antitumor drug (chlorin e6) in the core/shell interface of the nanoparticles. The experimental results demonstrated that the Y-shaped ligands on the nanoparticles enabled a significant enhancement of in vitro/in vivo cellular uptake for human primary glioblastoma U87-MG cells as a result of multivalent endocytosis by Y-shaped ligands (transferrin receptor-mediated endocytosis and the following Tat peptide-mediated cellular interaction). Furthermore, the Ce6-loaded nanoparticles showed significant enhancement of in vitro/in vivo photodynamic U87-MG cell ablation under light illumination.
Key Findings
1
Chlorin e6-loaded nanoparticles produced significantly enhanced in vitro and in vivo photodynamic ablation of U87-MG cells under light illumination.
2
Iron oxide nanoparticles were stabilized with Pluronic F127 and noncovalently functionalized with dopamine-terminated Y-shaped Tat peptide–transferrin ligands.
3
The Pluronic F127 coating enabled loading of chlorin e6 at the nanoparticle core–shell interface.
4
Y-shaped Tat/transferrin ligands significantly enhanced in vitro and in vivo uptake by human glioblastoma U87-MG cells through multivalent endocytosis.
Research Object
Iron oxide nanoparticles functionalized with dopamine-terminated Y-shaped Tat peptide/transferrin ligands and loaded with chlorin e6 for glioblastoma targeting
Research Subject
Multivalent ligand-mediated cellular uptake and photodynamic ablation of U87-MG glioblastoma cells, including transferrin receptor-mediated endocytosis and Tat peptide-mediated interaction
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2016-01-01
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