DNA sequencing with chain-terminating inhibitors
Секвенирование ДНК с использованием ингибиторов, терминирующих цепь
1977-12-01
SCID: 54.1/gjabry6g
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DNA polymeraseDNA sequencingbacteriophage φX174chain-terminating inhibitorsdideoxynucleoside triphosphates
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Abstract (AI)
A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.
Key Findings
1
Introduces a DNA sequencing method using 2′,3′-dideoxy and arabinonucleoside analogues as specific chain-terminating inhibitors of DNA polymerase.
2
The method is conceptually similar to the Sanger–Coulson plus-and-minus sequencing approach but uses chain termination to determine nucleotide sequences.
3
The method is reported to be faster and more accurate than both the plus and minus sequencing methods.
4
The technique was successfully applied to sequencing bacteriophage φX174 DNA.
Research Object
bacteriophage φX174 DNA
Research Subject
nucleotide sequence determination, including the method’s speed and accuracy using chain-terminating nucleoside analogues
Publication Details
Publication Date
1977-12-01
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