Drought-tolerant plant growth promoting<i>Bacillus</i>spp.: effect on growth, osmolytes, and antioxidant status of maize under drought stress
Устойчивые к засухе ризобактерии Bacillus spp., стимулирующие рост растений: влияние на рост, осмолиты и антиоксидантный статус кукурузы в условиях засухи
2010-12-01
SCID: 54.1/grnjg9vs
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antioxidant enzymesdrought-tolerant Bacillusmaize drought stressosmolyte accumulationplant growth-promoting bacteria
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Abstract (AI)
In present study Bacillus spp. screened for drought tolerance could tolerate minimal water potential (-0.73 MPa) were evaluated for plant growth promoting properties at –0.73 MPa. Drought stress affected growth of isolates as indicated by increased intracellular free amino acids, proline, total soluble sugars, and exopolysaccharides. Drought-tolerant Bacillus spp. HYD-B17, HYTAPB18, HYDGRFB19, BKB30, RMPB44 identified as Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus thuringiensis, Paenibacillus favisporus, Bacillus subtilis based on 16S rDNA gene sequence were used to study the effect of inoculation on growth, osmolytes, antioxidant status. Inoculation increased plant biomass, relative water content, leaf water potential, root adhering soil/root tissue ratio, aggregate stability, decreasing leaf water loss. Bacillus spp. effect on osmoregulation increased proline, sugars, free amino acids and decreased electrolyte leakage. Inoculation reduced the activity of antioxidant enzymes ascorbate peroxidase, catalase, glutathione peroxidase. As a result, Bacillus spp. inoculated maize seedlings showed physiological response that could alleviate drought stress negative effects.
Key Findings
1
Bacterial inoculation enhanced maize osmoregulation by increasing proline, soluble sugars, and free amino acids while reducing leaf water loss and electrolyte leakage.
2
Drought stress increased bacterial intracellular free amino acids, proline, total soluble sugars, and exopolysaccharide production.
3
Five drought-tolerant bacterial isolates—identified as Bacillus amyloliquefaciens, B. licheniformis, B. thuringiensis, Paenibacillus favisporus, and B. subtilis—tolerated a minimum water potential of −0.73 MPa.
4
Inoculated maize showed reduced ascorbate peroxidase, catalase, and glutathione peroxidase activities, indicating that Bacillus-associated physiological changes alleviated drought stress effects.
5
Inoculating maize with drought-tolerant Bacillus spp. increased plant biomass, relative water content, leaf water potential, root adhering soil-to-root tissue ratio, and soil aggregate stability.
Research Object
Maize seedlings inoculated with drought-tolerant plant growth-promoting Bacillus spp. under drought stress
Research Subject
Effects of Bacillus inoculation on maize growth, water status, osmolyte accumulation, electrolyte leakage, and antioxidant enzyme activity under drought stress
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2010-12-01
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