A transmembrane protein confers the Yr26/Yr24/YrCH42/YrG22-mediated stripe rust resistance in wheat

Xiaojie Wang; Zhensheng Kang; Jaroslav Doležel; Evans Lagudah; Jianhui Wu; Qingdong Zeng; Xiaoting Wang; John P. Rathjen; Aizhong Cao; Peidu Chen; Shengjie Liu; Keqing Mu; Shuqing Yang; Mingjie Xiang; Xiangrui Cheng; Yibo Zhang; Ying Zhao; Jiangna Han; Shuo Huang; Haitao Dong; Jiwen Zhao; Fengping Yuan; R. K. Sharma; Michael Norman; Weijun Zheng; Chunlian Li; Kateřina HolušováJ; Sambasivam Periyannan; Dejun Han

doi:10.1038/s41467-026-76091-5

A transmembrane protein confers the Yr26/Yr24/YrCH42/YrG22-mediated stripe rust resistance in wheat

Трансмембранный белок обеспечивает устойчивость пшеницы к полосатой ржавчине, опосредованную генами Yr26/Yr24/YrCH42/YrG22

Xiaojie Wang, Zhensheng Kang, Jaroslav Doležel, Evans Lagudah, Jianhui Wu, Qingdong Zeng, Xiaoting Wang, John P. Rathjen, Aizhong Cao, Peidu Chen, Shengjie Liu, Keqing Mu, Shuqing Yang, Mingjie Xiang, Xiangrui Cheng, Yibo Zhang, Ying Zhao, Jiangna Han, Shuo Huang, Haitao Dong, Jiwen Zhao, Fengping Yuan, R. K. Sharma, Michael Norman, Weijun Zheng, Chunlian Li, Kateřina HolušováJ, Sambasivam Periyannan, Dejun Han

2026-08-24

SCID: 54.1/hzf99zap Discuss with AI

Ca2+-dependent cell deathPuccinia striiformis f. sp. triticiYr26 stripe rust resistance genemap-based cloningtransmembrane protein

Abstract (AI)

Abstract Stripe or yellow rust (YR), caused by Puccinia striiformis Westend. f. sp. tritici ( Pst ), is a devastating fungal disease of wheat. Here, we report the map-based cloning of Yr26 (synonyms: Yr24 , YrCH42 , and YrG22 ), a major stripe rust resistance gene, and its validation by gene silencing, mutation analysis and transgenic complementation. Yr26 , which firstly arises in emmer wheat and recently has introgressed into bread wheat, is a newly evolved gene encoding a transmembrane protein. AlphaFold v3.0 predictes a C-terminal four-helical bundle domain that is critical for triggering Ca 2+ -dependent cell death, a hallmark of the hypersensitive response. The cloning of Yr26 offers not only a genetic resource for pyramiding stripe rust resistance genes in wheat breeding but also an opportunity to study host–pathogen co-evolution.

Key Findings

Cloning Yr26 provides a genetic resource for pyramiding stripe rust resistance genes and studying wheat–Pst host–pathogen co-evolution.

Yr26 encodes a transmembrane protein whose predicted C-terminal four-helical bundle is critical for triggering Ca2+-dependent cell death and the hypersensitive response.

Yr26 is a newly evolved stripe rust resistance gene that originated in emmer wheat and was recently introgressed into bread wheat.

Yr26, synonymous with Yr24, YrCH42, and YrG22, was map-based cloned and validated through gene silencing, mutation analysis, and transgenic complementation.

Research Object

Yr26/Yr24/YrCH42/YrG22-mediated stripe rust resistance in wheat

Research Subject

The molecular identity, transmembrane-protein function, and Ca2+-dependent hypersensitive cell-death mechanism of the Yr26-mediated resistance

Publication Details

DOI

10.1038/s41467-026-76091-5

Publication Date

2026-08-24

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https://www.nature.com/articles/s41467-026-7609…

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Authors

Xiaojie Wang

Zhensheng Kang

Jaroslav Doležel

Evans Lagudah

Jianhui Wu

Qingdong Zeng

Xiaoting Wang

John P. Rathjen

Aizhong Cao

Peidu Chen

Shengjie Liu

Keqing Mu

Shuqing Yang

Mingjie Xiang

Xiangrui Cheng

Yibo Zhang

Ying Zhao

Jiangna Han

Shuo Huang

Haitao Dong

Jiwen Zhao

Fengping Yuan

R. K. Sharma

Michael Norman

Weijun Zheng

Chunlian Li

Kateřina HolušováJ

Sambasivam Periyannan

Dejun Han

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