Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay

Эффективное и масштабируемое получение примордиальных герминативных клеток в 2D-культуре с использованием покрытия из экстракта базальной мембраны
Manuel A.F.V. Gonçalves, Hailiang Mei, Susana M. Chuva de Sousa Lopes, Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia Van Der Helm, Valérie F. Van Der Schrier, Christian Freund
2023-05-23
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2D culturebasement membrane extract overlayefficient generationprimordial germ cellsscalable generation
Abstract (AI)
gametogenesis.
Key Findings
1
A 2D culture method using a basement membrane extract overlay enables efficient and scalable generation of primordial germ cells.
2
The basement membrane extract overlay approach supports gametogenesis-related cell differentiation in vitro.
3
The described protocol improves scalability of primordial germ cell production compared to unspecified alternatives.
Research Object

primordial germ cells generated in 2D culture with basement membrane extract overlay

Research Subject

efficient and scalable generation protocol for primordial germ cells using a basement membrane extract overlay in 2D culture (efficiency, scalability, and culture conditions enabling gametogenesis-related differentiation)

Publication Details
Publication Date
2023-05-23
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29
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Author Information
Authors
Manuel A.F.V. Gonçalves
Hailiang Mei
Susana M. Chuva de Sousa Lopes
Arend W. Overeem
Yolanda W. Chang
Ioannis Moustakas
Celine M. Roelse
Sanne Hillenius
Talia Van Der Helm
Valérie F. Van Der Schrier
Christian Freund
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