Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

Rob Knight; Bruce W. Birren; William Nelson; Philip Hugenholtz; Gene W. Tyson; Thomas Rattei; Frank Oliver Glöckner; Noah Fierer; Steven Hallam; Natalia Ivanova; Nikos C. Kyrpides; Brett J. Baker; Jillian F. Banfield; Donovan H. Parks; Susannah G. Tringe; Thijs J. G. Ettema; Rex R. Malmstrom; Tanja Woyke; A. Murat Eren; George M Garrity; Robert M. Bowers; Jonathan A. Eisen; Alla Lapidus; Ramūnas Stepanauskas; Pelin Yilmaz; Peer Bork; Eric D. Becraft; Christian Rinke; Brian P. Hedlund; Adam R. Rivers; Jack A. Gilbert; Sean P. Jungbluth; Folker Meyer; ROBERT FINN; Alicia Clum; Guy Cochrane; Frederik Schulz; George M. Weinstock; Ilene Karsch‐Mizrachi; T. B. K. Reddy; Emiley A. Eloe‐Fadrosh; Mircea Podar; Lynn M. Schriml; Shibu Yooseph; Katherine D. McMahon; Wen‐Tso Liu; Alex Copeland; Jeremy A. Dodsworth; Scott Tighe; Miranda Harmon‐Smith; Devin F. R. Doud; Jessica K. Jarett; Granger Sutton; Konstantinos T. Konstantinidis; Gene W Tyson

doi:10.1038/nbt.3893

Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

Минимальная информация о единичном амплифицированном геноме (MISAG) и о геноме, собранном из метагенома (MIMAG) бактерий и архей

Rob Knight, Bruce W. Birren, William Nelson, Philip Hugenholtz, Gene W. Tyson, Thomas Rattei, Frank Oliver Glöckner, Noah Fierer, Steven Hallam, Natalia Ivanova, Nikos C. Kyrpides, Brett J. Baker, Jillian F. Banfield, Donovan H. Parks, Susannah G. Tringe, Thijs J. G. Ettema, Rex R. Malmstrom, Tanja Woyke, A. Murat Eren, George M Garrity, Robert M. Bowers, Jonathan A. Eisen, Alla Lapidus, Ramūnas Stepanauskas, Pelin Yilmaz, Peer Bork, Eric D. Becraft, Christian Rinke, Brian P. Hedlund, Adam R. Rivers, Jack A. Gilbert, Sean P. Jungbluth, Folker Meyer, ROBERT FINN, Alicia Clum, Guy Cochrane, Frederik Schulz, George M. Weinstock, Ilene Karsch‐Mizrachi, T. B. K. Reddy, Emiley A. Eloe‐Fadrosh, Mircea Podar, Lynn M. Schriml, Shibu Yooseph, Katherine D. McMahon, Wen‐Tso Liu, Alex Copeland, Jeremy A. Dodsworth, Scott Tighe, Miranda Harmon‐Smith, Devin F. R. Doud, Jessica K. Jarett, Granger Sutton, Konstantinos T. Konstantinidis, Gene W Tyson

2017-08-01

SCID: 54.1/mjyxduv2 Discuss with AI

Minimum Information about Any (x) Sequence (MIxS)Minimum Information about a Metagenome-Assembled Genome (MIMAG)Minimum Information about a Single Amplified Genome (MISAG)assembly qualitygenome completeness and contamination

Abstract (AI)

We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.

Key Findings

Community-wide adoption of MISAG and MIMAG is expected to facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.

MISAG and MIMAG extend the MIxS framework and specify reporting requirements including assembly quality and estimates of genome completeness and contamination.

The Genomic Standards Consortium developed two standards: MISAG (Single Amplified Genome) and MIMAG (Metagenome-Assembled Genome) for bacterial and archaeal genomes.

These standards are compatible with other GSC checklists (MIGS, MIMS, MIMARKS) and can be used in combination with them.

Research Object

The MISAG and MIMAG reporting standards for bacterial and archaeal genome sequences

Research Subject

Specification of minimum metadata and quality metrics for single amplified genomes and metagenome-assembled genomes, including assembly quality, genome completeness and contamination estimates

Publication Details

DOI

10.1038/nbt.3893

Publication Date

2017-08-01

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Publisher

ISSN

Open access PDF

https://www.nature.com/articles/nbt.3893.pdf

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Author Information

Authors

Rob Knight

Bruce W. Birren

William Nelson

Philip Hugenholtz

Gene W. Tyson

Thomas Rattei

Frank Oliver Glöckner

Noah Fierer

Steven Hallam

Natalia Ivanova

Nikos C. Kyrpides

Brett J. Baker

Jillian F. Banfield

Donovan H. Parks

Susannah G. Tringe

Thijs J. G. Ettema

Rex R. Malmstrom

Tanja Woyke

A. Murat Eren

George M Garrity

Robert M. Bowers

Jonathan A. Eisen

Alla Lapidus

Ramūnas Stepanauskas

Pelin Yilmaz

Peer Bork

Eric D. Becraft

Christian Rinke

Brian P. Hedlund

Adam R. Rivers

Jack A. Gilbert

Sean P. Jungbluth

Folker Meyer

ROBERT FINN

Alicia Clum

Guy Cochrane

Frederik Schulz

George M. Weinstock

Ilene Karsch‐Mizrachi

T. B. K. Reddy

Emiley A. Eloe‐Fadrosh

Mircea Podar

Lynn M. Schriml

Shibu Yooseph

Katherine D. McMahon

Wen‐Tso Liu

Alex Copeland

Jeremy A. Dodsworth

Scott Tighe

Miranda Harmon‐Smith

Devin F. R. Doud

Jessica K. Jarett

Granger Sutton

Konstantinos T. Konstantinidis

Gene W Tyson

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References available in scid.ai1

The Human Microbiome Project2007

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