Telomere measurement by quantitative PCR
Измерение теломер методом количественной ПЦР
2002-05-15
SCID: 54.1/n7bdkygd
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TTAGGG repeatsfluorescence-based assayquantitative PCRtelomere measurementvertebrate DNA
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Abstract (AI)
It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a primer pair that eliminates this problem, allowing simple and rapid measurement of telomeres in a closed tube, fluorescence-based assay. This assay will facilitate investigations of the biology of telomeres and the roles they play in the molecular pathophysiology of diseases and aging.
Key Findings
1
PCR amplification of vertebrate telomeres was previously considered impossible because TTAGGG- and CCCTAA-targeting primers were expected to generate only primer-dimer products.
2
The assay is intended to facilitate research on telomere biology and their roles in disease pathophysiology and aging.
3
The resulting assay provides a simple, rapid, closed-tube, fluorescence-based method for measuring telomeres.
4
The study introduces a primer pair that eliminates primer-dimer interference and enables telomere measurement by quantitative PCR.
Research Object
vertebrate DNA telomeres
Research Subject
quantitative PCR measurement of telomere length using a closed-tube fluorescence-based assay
Publication Details
Publication Date
2002-05-15
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