Stem cells: past, present, and future

Стволовые клетки: прошлое, настоящее и будущее
Wojciech Zakrzewski, Maciej Dobrzyński, Maria Szymonowicz, Zbigniew Rybak
2019-02-26

controlled stem cell culturingextracellular vesicle-based therapiesgraphene scaffoldsstem cell therapyteratoma formation assays
In recent years, stem cell therapy has become a very promising and advanced scientific research topic. The development of treatment methods has evoked great expectations. This paper is a review focused on the discovery of different stem cells and the potential therapies based on these cells. The genesis of stem cells is followed by laboratory steps of controlled stem cell culturing and derivation. Quality control and teratoma formation assays are important procedures in assessing the properties of the stem cells tested. Derivation methods and the utilization of culturing media are crucial to set proper environmental conditions for controlled differentiation. Among many types of stem tissue applications, the use of graphene scaffolds and the potential of extracellular vesicle-based therapies require attention due to their versatility. The review is summarized by challenges that stem cell therapy must overcome to be accepted worldwide. A wide variety of possibilities makes this cutting edge therapy a turning point in modern medicine, providing hope for untreatable diseases.
1
Derivation methods and culture media are crucial for establishing controlled environmental conditions that direct stem cell differentiation.
2
Global acceptance of stem cell therapy remains dependent on overcoming significant challenges, despite its potential for treating currently untreatable diseases.
3
Graphene scaffolds and extracellular vesicle-based therapies are highlighted as versatile and promising stem-cell application areas.
4
Quality control and teratoma formation assays are identified as important procedures for evaluating stem cell properties.
5
The review traces stem cell discovery and genesis, alongside laboratory methods for controlled stem cell culturing and derivation.

stem cells and stem cell-based therapies

the discovery, derivation, culturing, quality assessment, controlled differentiation, applications, and clinical challenges of stem cells and stem cell-based therapies

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2019-02-26
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Authors
Wojciech Zakrzewski
Maciej Dobrzyński
Maria Szymonowicz
Zbigniew Rybak
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