Mammalian cell transfection: the present and the future
Трансфекция клеток млекопитающих: настоящее и будущее
2010-06-11
SCID: 54.1/nax2998u
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laser-microscope systemmRNA transfectionmammalian cell transfectionnucleic acid deliverypoint-directed transfection
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Abstract (AI)
Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical. These methods have advanced to make it possible to deliver nucleic acids to specific subcellular regions of cells by use of a precisely controlled laser-microscope system. The combination of point-directed transfection and mRNA transfection is a new way of studying the function of genes and gene products. However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.
Key Findings
1
Advances in precisely controlled laser-microscope systems enable nucleic acid delivery to specific subcellular regions.
2
Combining point-directed transfection with mRNA transfection provides a new approach for studying gene and gene-product function.
3
Mammalian cell transfection methods are classified into biological, chemical, and physical approaches.
4
No transfection method is universally optimal; selection depends on each method’s advantages, limitations, experimental design, and objective.
5
Transfection enables functional analysis of genes and gene products in mammalian cells.
Research Object
mammalian cell transfection methods
Research Subject
methods for delivering nucleic acids into mammalian cells and their application to studying gene and gene-product function, including advantages, disadvantages, and optimization for experimental objectives
Publication Details
Publication Date
2010-06-11
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