Oxidase‐Like Activity of Polymer‐Coated Cerium Oxide Nanoparticles
Оксидазоподобная активность покрытых полимером наночастиц оксида церия
2009-01-07
SCID: 54.1/pkzh5v6a
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TMB colorimetric detectioncerium oxide nanoparticlesenzyme-linked immunosorbent assayoxidase-like activitypolymer-coated nanoparticles
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Abstract (AI)
Inorganic enzyme? Ceria nanoparticles exhibit unique oxidase-like activity at acidic pH values. These redox catalysts can be used in immunoassays (ELISA) when modified with targeting ligands (see picture; light blue and yellow structures are nanoparticles with attached ligands). This modification allows both for binding and for detection by the catalytic oxidation of sensitive colorimetric dyes (e.g. TMB).
Key Findings
1
Cerium oxide nanoparticles exhibit oxidase-like redox catalytic activity, particularly under acidic pH conditions.
2
Nanoparticle-mediated oxidation of colorimetric substrates such as TMB provides a detectable assay signal.
3
Polymer coating and attachment of targeting ligands enable cerium oxide nanoparticles to combine specific binding with catalytic detection.
4
The modified nanoparticles can be applied in ELISA immunoassays as inorganic enzyme-like labels.
Research Object
polymer-coated cerium oxide nanoparticles
Research Subject
oxidase-like activity and its use for catalytic colorimetric detection in ELISA under acidic conditions
Publication Details
Publication Date
2009-01-07
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