Species identification of aphids (Insecta: Hemiptera: Aphididae) through DNA barcodes
Идентификация видов тлей (Insecta: Hemiptera: Aphididae) с помощью ДНК-штрихкодов
2008-10-29
SCID: 54.1/qzwfpry8
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DNA barcodingaphid species identificationcytochrome c oxidase 1mitochondrial DNAparthenogenetic reproduction
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Abstract (AI)
A 658-bp fragment of mitochondrial DNA from the 5' region of the mitochondrial cytochrome c oxidase 1 (COI) gene has been adopted as the standard DNA barcode region for animal life. In this study, we test its effectiveness in the discrimination of over 300 species of aphids from more than 130 genera. Most (96%) species were well differentiated, and sequence variation within species was low, averaging just 0.2%. Despite the complex life cycles and parthenogenetic reproduction of aphids, DNA barcodes are an effective tool for identification.
Key Findings
1
A 658-bp fragment from the mitochondrial COI gene was evaluated as a DNA barcode for identifying more than 300 aphid species across over 130 genera.
2
Despite aphids’ complex life cycles and parthenogenetic reproduction, DNA barcodes effectively support species identification.
3
Intraspecific sequence variation was low, averaging only 0.2%.
4
The COI barcode successfully differentiated 96% of the examined aphid species.
Research Object
Aphids (Insecta: Hemiptera: Aphididae), representing over 300 species from more than 130 genera
Research Subject
The effectiveness of the 658-bp mitochondrial COI DNA barcode region for species discrimination and identification, including interspecific differentiation and intraspecific sequence variation
Publication Details
Publication Date
2008-10-29
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