Differential Genetic Strategies of Burkholderia vietnamiensis and Paraburkholderia kururiensis for Root Colonization of Oryza sativa subsp. japonica and O. sativa subsp. indica , as Revealed by Transposon Mutagenesis Sequencing

Различия в генетических стратегиях Burkholderia vietnamiensis и Paraburkholderia kururiensis при колонизации корней Oryza sativa subsp. japonica и O. sativa subsp. indica, выявленные методом секвенирования транспозонных мутантов
Adrian Wallner, Nicolas Busset, Joy Lachat, Ludivine Guigard, Eoghan King, Isabelle Rimbault, Peter Mergaert, Gilles Béna, Lionel Moulin
2022-07-06

Burkholderia vietnamiensisParaburkholderia kururiensisrice varietiesroot colonizationtransposon sequencing
genus includes both beneficial and phytopathogenic species, as well as species able to colonize animal hosts and cause disease in humans. We need to understand to what extent the bacterial strategies used for the different biotic interactions differ depending on the host and if strains with agricultural potential could also pose a threat toward other plant hosts or humans. To start answering these questions, we used in this study transposon sequencing to identify genetic traits in Burkholderia vietnamiensis and Paraburkholderia kururiensis that contribute to the colonization of two different rice varieties. Our results revealed large differences in the fitness gene sets between the two strains and between the host plants, suggesting a strong specificity in each bacterium-plant interaction.
1
Host plant variety strongly influenced the genetic requirements for bacterial colonization, indicating specific bacterium–plant interactions.
2
The findings highlight distinct genetic strategies underlying interactions with Oryza sativa subsp. japonica and O. sativa subsp. indica.
3
The two bacterial strains exhibited substantially different sets of genes affecting fitness during rice colonization.
4
Transposon sequencing identified genetic traits contributing to root colonization of two rice varieties by Burkholderia vietnamiensis and Paraburkholderia kururiensis.

Burkholderia vietnamiensis and Paraburkholderia kururiensis colonizing Oryza sativa subsp. japonica and Oryza sativa subsp. indica rice plants

Strain- and host-specific genetic traits and fitness gene sets underlying bacterial root colonization

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Publication Date
2022-07-06
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Adrian Wallner
Nicolas Busset
Joy Lachat
Ludivine Guigard
Eoghan King
Isabelle Rimbault
Peter Mergaert
Gilles Béna
Lionel Moulin
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