Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
Минимальная информация для исследований внеклеточных везикул (MISEV2023): от базовых до передовых подходов
2024-02-01
SCID: 54.1/vfn9u87a
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EV characterizationEV metrologyEV nomenclatureEV release and uptakeextracellular vesicles
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Abstract (AI)
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.
Key Findings
1
Compiled from ISEV expert task forces and feedback from more than 1000 researchers, MISEV2023 aims to improve reproducibility and accelerate robust EV discoveries.
2
MISEV2023 emphasizes persistent challenges in EV nomenclature, separation from non-vesicular particles, characterization, and functional evaluation.
3
MISEV2023 updates the 2014 and 2018 guidelines for rigorous extracellular vesicle research across basic and clinical applications.
4
The document reviews available methods for EV production, separation, and characterization from cell cultures, body fluids, and solid tissues.
5
The guideline summarizes advanced techniques, EV release and uptake mechanisms, and emerging in vivo approaches, while discussing methodological advantages and limitations.
Research Object
Extracellular vesicles (EVs) from multiple sources (cell culture, body fluids, solid tissues)
Research Subject
Available approaches, advantages, limitations, and advanced methods for EV production, separation, characterization, release, uptake, and in vivo study
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2024-02-01
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