Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

Nicolò Fusi, Craig B. Wilen, David E. Root, John G. Doench, Herbert W. Virgin, Katherine Donovan, Mudra Hegde, Emma W Vaimberg, Meagan E. Sullender, Jennifer Listgarten, Zuzana Tóthová, Ian C. P. Smith, Robert C. Orchard

2016-01-18

SCID: 54.1/yfn5s8r7

Publication Details

DOI

10.1038/nbt.3437

Publication Date

2016-01-18

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Author Information

Authors

Nicolò Fusi

Craig B. Wilen

David E. Root

John G. Doench

Herbert W. Virgin

Katherine Donovan

Mudra Hegde

Emma W Vaimberg

Meagan E. Sullender

Jennifer Listgarten

Zuzana Tóthová

Ian C. P. Smith

Robert C. Orchard

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