Isolation and characterization of fungi and bacteria able to grow on media containing gasoline and diesel fuel

Petroleum products are significant environmental pollutants. This study aimed to isolate microorganisms able to grow on media containing gasoline and diesel fuel. Microorganisms were isolated from soils sampled near gasoline and diesel pumps. Bacterial isolates were characterized and tested on media containing concentrations ranging from 10 to 100 % gasoline and diesel fuel and combinations of both 50/50 % and 25/25/50 % (gasoline/ diesel/ Mueller Hinton broth). Results showed that microbial isolates belong to the genera Pseudomonas, Bacillus, Staphylococcus, Micrococcus, Flavobacterium, Actinobacteria, Penicillium, Hansfordia and Alternaria. Pseudomonas spp. and Bacillus spp. showed the ability to grow on both products up to the concentration of 80 %. However, no growth was noticed above that concentration and on both mixtures. Throughout this study, it has been shown that using a selective screening method for microorganisms able to grow on pollutants can present a significant advantage for bioremediation. Keywords: Pollution; gasoline; diesel; microorganisms.