GIInger predicts homologous recombination deficiency and patient response to PARPi treatment from shallow genomic profiles

Philipp Harter, Christoph Grimm, Nicoletta Colombo, Zhenyu Xu, Nicola Normanno, Rieke Kempfer, Ana Claudia Marques, Ignace Vergote, Ewan St. John Smith, Isabelle Ray‐Coquard, Antonio González-Martı́n, Sandro Pignata, Keiichi Fujiwara, Éric Pujade-Lauraine, Pierre‐Alexandre Just, Pierre Saintigny, Amjad Alkodsi, Leonhard Müllauer, Massimo Barberis, Alexandre Harlé, Cristin Roma, Davide Vacirca, Adrien Buisson, Pauline Gilson, Christian Pozzorini, Gregoire Andre, Tommaso Coletta, Jonathan Bieler, Loïc Ferrer, Alexandra Saitta, Floriane Consales Barras, Lucia Ripol, Martin Fritzsche, Ray M. Marín, Ursula Denison, Adrian Willig
2023-12-01

SCID:  54.1/zrq4c9x3
Homologous recombination deficiency (HRD) is a predictive biomarker for poly(ADP-ribose) polymerase 1 inhibitor (PARPi) sensitivity. Routine HRD testing relies on identifying BRCA mutations, but additional HRD-positive patients can be identified by measuring genomic instability (GI), a consequence of HRD. However, the cost and complexity of available solutions hamper GI testing. We introduce a deep learning framework, GIInger, that identifies GI from HRD-induced scarring observed in low-pass whole-genome sequencing data. GIInger seamlessly integrates into standard BRCA testing workflows and yields reproducible results concordant with a reference method in a multisite study of 327 ovarian cancer samples. Applied to a BRCA wild-type enriched subgroup of 195 PAOLA-1 clinical trial patients, GIInger identified HRD-positive patients who experienced significantly extended progression-free survival when treated with PARPi. GIInger is, therefore, a cost-effective and easy-to-implement method for accurately stratifying patients with ovarian cancer for first-line PARPi treatment.
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2023-12-01
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Authors
Philipp Harter
Christoph Grimm
Nicoletta Colombo
Zhenyu Xu
Nicola Normanno
Rieke Kempfer
Ana Claudia Marques
Ignace Vergote
Ewan St. John Smith
Isabelle Ray‐Coquard
Antonio González-Martı́n
Sandro Pignata
Keiichi Fujiwara
Éric Pujade-Lauraine
Pierre‐Alexandre Just
Pierre Saintigny
Amjad Alkodsi
Leonhard Müllauer
Massimo Barberis
Alexandre Harlé
Cristin Roma
Davide Vacirca
Adrien Buisson
Pauline Gilson
Christian Pozzorini
Gregoire Andre
Tommaso Coletta
Jonathan Bieler
Loïc Ferrer
Alexandra Saitta
Floriane Consales Barras
Lucia Ripol
Martin Fritzsche
Ray M. Marín
Ursula Denison
Adrian Willig
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