Laccase engineering by rational and evolutionary design
Инженерия лакказ с использованием рационального и эволюционного дизайна
2015-01-13
SCID: 54.1/b3vauf2e
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directed evolutionlaccase engineeringmulticopper oxidasesrational designsite-directed mutagenesis
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Abstract (AI)
Laccases are considered as green catalysts of great biotechnological potential. This has attracted a great interest in designing laccases a la carte with enhanced stabilities or activities tailored to specific conditions for different fields of application. Over 20 years, numerous efforts have been taken to engineer these multicopper oxidases and to understand their reaction mechanisms by site-directed mutagenesis, and more recently, using computational calculations and directed evolution tools. In this work, we review the most relevant contributions made in the field of laccase engineering, from the comprehensive study of their structure-function relationships to the tailoring of outstanding biocatalysts.
Key Findings
1
Computational calculations and directed evolution have more recently expanded the tools available for engineering laccases.
2
Laccase engineering has targeted enhanced stability and catalytic activity under application-specific conditions.
3
Laccases are green catalysts with substantial biotechnological potential, motivating efforts to tailor their properties for diverse applications.
4
More than two decades of research have used site-directed mutagenesis to investigate laccase mechanisms and structure–function relationships.
5
The field has progressed from fundamental structure–function studies toward the development of high-performing, application-tailored laccase biocatalysts.
Research Object
laccases (multicopper oxidases)
Research Subject
engineering strategies and structure–function relationships used to tailor laccase stability, activity, reaction mechanisms, and biocatalytic performance for specific conditions
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2015-01-13
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