Re-designing the substrate binding pocket of laccase for enhanced oxidation of sinapic acid
Перепроектирование кармана связывания субстрата лакказы для усиления окисления синаповой кислоты
2015-12-29
SCID: 54.1/jbwnzrn2
Discuss with AI
high redox potentialiterative saturation mutagenesislaccasesinapic acid oxidationsubstrate binding pocket
Figures from the paper
Abstract (AI)
Iterative saturation mutagenesis was performed over six residues delimiting the substrate binding pocket of a high redox potential chimeric laccase with the aim of enhancing its activity over sinapic acid, a lignin-related phenol of industrial interest.
Key Findings
1
Iterative saturation mutagenesis targeted six residues delimiting the substrate-binding pocket of a high-redox-potential chimeric laccase.
2
The abstract describes substrate-pocket redesign but provides no specific mutations, activity improvements, or comparative performance data.
3
The engineering strategy aimed to enhance laccase oxidation activity toward sinapic acid, an industrially relevant lignin-related phenol.
Research Object
high-redox-potential chimeric laccase substrate-binding pocket
Research Subject
enhanced catalytic oxidation of sinapic acid through substrate-binding pocket redesign
Publication Details
Publication Date
2015-12-29
Journal
Publisher
ISSN
Open access PDF
Access Type
Author Information
Download PDF
Subscribe to digest