Comprehensive genomic characterization of squamous cell lung cancers

Комплексная геномная характеристика плоскоклеточного рака лёгкого
Eric S. Lander, Stacey Gabriel, Marco A. Marra, Robert A. Holt, Angela Tam, Miruna Balasundaram, Haiyan I. Li, Richard A. Moore, Eric Chuah, Michael Mayo, Jacqueline E. Schein, Andrew J. Mungall, A. Gordon Robertson, Nina Thiessen, Matthew Meyerson, Nils Gehlenborg, Kristian Cibulskis, Andrew D. Cherniack, Michael S. Lawrence, Doug Voet, Gad Getz, Lynda Chin, Pei Lin, Rameen Beroukhim, Gordon Saksena, Juok Cho, Hailei Zhang, Aaron McKenna, Chang-Jiun Wu, Stephen E. Schumacher, Petar Stojanov, Sachet Shukla, Nam H. Pho, Helga Thorvaldsdottir, Barbara Tabak, Andrey Sivachenko, Marc-Danie Nazaire, Michael Noble, Lihua Zou, Jim Robinson, Jill Mesirov, Douglas Voet, Marcin Imielinski, Peter S. Hammerman, Huy Nguyen, Wendy Winckler, Andrew Crenshaw, Elena Helman, Spring Yingchun Liu, Scott L. Carter, Daniel DiCara, Rui Jing, Bryan Hernandez, Bruce E. Johnson, David Kwiatkowski, Bruce Johnson, Carrie Sougnez, Kristin Ardlie, Robert C. Onofrio, Yongjun Zhao, Karen Mungall, Andy Chu, An He, Elizabeth Chun, Richard Varhol, Carrie Hirst, Martin Hirst, Robin J. N. Coope, Hye-Jung E. Chun, Dominik Stoll, Yaron S. N. Butterfield, Ka Ming Nip, Erin Pleasance, Jared R. Slobodan, Ranabir Guin, Gordon Robertson, Andy Mungall, Darlene Lee, Thomas Zeng, Richard Corbett
2012-09-07

TP53 mutationsThe Cancer Genome Atlasepigenomic alterationsgenomic alterationslung squamous cell carcinoma
Lung squamous cell carcinoma is a common type of lung cancer, causing approximately 400,000 deaths per year worldwide. Genomic alterations in squamous cell lung cancers have not been comprehensively characterized, and no molecularly targeted agents have been specifically developed for its treatment. As part of The Cancer Genome Atlas, here we profile 178 lung squamous cell carcinomas to provide a comprehensive landscape of genomic and epigenomic alterations. We show that the tumour type is characterized by complex genomic alterations, with a mean of 360 exonic mutations, 165 genomic rearrangements, and 323 segments of copy number alteration per tumour. We find statistically recurrent mutations in 11 genes, including mutation of TP53 in nearly all specimens. Previously unreported loss-of-function mutations are seen in the HLA-A class I major histocompatibility gene. Significantly altered pathways included NFE2L2 and KEAP1 in 34%, squamous differentiation genes in 44%, phosphatidylinositol-3-OH kinase pathway genes in 47%, and CDKN2A and RB1 in 72% of tumours. We identified a potential therapeutic target in most tumours, offering new avenues of investigation for the treatment of squamous cell lung cancers. Comprehensive analyses of 178 lung squamous cell carcinomas by The Cancer Genome Atlas project show that the tumour type is characterized by complex genomic alterations, with statistically recurrent mutations in 11 genes, including TP53 in nearly all samples; a potential therapeutic target is identified in most of the samples studied. The Cancer Genome Atlas consortium has analysed 178 lung squamous cell carcinomas, a common type of lung cancer for which comprehensive genomic analyses have not previously been available. The researchers report that this tumour type is characterized by complex genomic alterations, with recurrent mutations in 18 genes, including TP53 in nearly all samples. They also report frequent mutations in squamous differentiation genes. Collectively, these analyses identify potential therapeutic targets worthy of further investigation.
1
A potential therapeutic target was identified in most tumors, providing new avenues for targeted treatment research despite the absence of specifically developed molecular therapies.
2
Major altered pathways included NFE2L2/KEAP1 in 34%, squamous differentiation genes in 44%, the phosphatidylinositol-3-OH kinase pathway in 47%, and CDKN2A/RB1 in 72% of tumors.
3
Statistically recurrent mutations occurred in 11 genes, with TP53 mutated in nearly all specimens; loss-of-function mutations were also identified in HLA-A.
4
The Cancer Genome Atlas profiled 178 lung squamous cell carcinomas, establishing a comprehensive genomic and epigenomic alteration landscape.
5
Tumors exhibited substantial genomic complexity, averaging 360 exonic mutations, 165 genomic rearrangements, and 323 copy-number alteration segments per tumor.

178 lung squamous cell carcinomas

The comprehensive landscape and recurrent patterns of genomic and epigenomic alterations, including mutations, genomic rearrangements, copy-number alterations, and pathway disruptions

Publication Details
Publication Date
2012-09-07
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Authors
Eric S. Lander
Stacey Gabriel
Marco A. Marra
Robert A. Holt
Angela Tam
Miruna Balasundaram
Haiyan I. Li
Richard A. Moore
Eric Chuah
Michael Mayo
Jacqueline E. Schein
Andrew J. Mungall
A. Gordon Robertson
Nina Thiessen
Matthew Meyerson
Nils Gehlenborg
Kristian Cibulskis
Andrew D. Cherniack
Michael S. Lawrence
Doug Voet
Gad Getz
Lynda Chin
Pei Lin
Rameen Beroukhim
Gordon Saksena
Juok Cho
Hailei Zhang
Aaron McKenna
Chang-Jiun Wu
Stephen E. Schumacher
Petar Stojanov
Sachet Shukla
Nam H. Pho
Helga Thorvaldsdottir
Barbara Tabak
Andrey Sivachenko
Marc-Danie Nazaire
Michael Noble
Lihua Zou
Jim Robinson
Jill Mesirov
Douglas Voet
Marcin Imielinski
Peter S. Hammerman
Huy Nguyen
Wendy Winckler
Andrew Crenshaw
Elena Helman
Spring Yingchun Liu
Scott L. Carter
Daniel DiCara
Rui Jing
Bryan Hernandez
Bruce E. Johnson
David Kwiatkowski
Bruce Johnson
Carrie Sougnez
Kristin Ardlie
Robert C. Onofrio
Yongjun Zhao
Karen Mungall
Andy Chu
An He
Elizabeth Chun
Richard Varhol
Carrie Hirst
Martin Hirst
Robin J. N. Coope
Hye-Jung E. Chun
Dominik Stoll
Yaron S. N. Butterfield
Ka Ming Nip
Erin Pleasance
Jared R. Slobodan
Ranabir Guin
Gordon Robertson
Andy Mungall
Darlene Lee
Thomas Zeng
Richard Corbett
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