A framework for human microbiome research
Рамки исследований человеческого микробиома
2012-06-01
SCID: 54.1/qc7nmnxx
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16S ribosomal RNAHuman Microbiome Projectmetagenomic protocolsmetagenomic sequencereference strains
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Abstract (AI)
A variety of microbial communities and their genes (the microbiome) exist throughout the human body, with fundamental roles in human health and disease. The National Institutes of Health (NIH)-funded Human Microbiome Project Consortium has established a population-scale framework to develop metagenomic protocols, resulting in a broad range of quality-controlled resources and data including standardized methods for creating, processing and interpreting distinct types of high-throughput metagenomic data available to the scientific community. Here we present resources from a population of 242 healthy adults sampled at 15 or 18 body sites up to three times, which have generated 5,177 microbial taxonomic profiles from 16S ribosomal RNA genes and over 3.5 terabases of metagenomic sequence so far. In parallel, approximately 800 reference strains isolated from the human body have been sequenced. Collectively, these data represent the largest resource describing the abundance and variety of the human microbiome, while providing a framework for current and future studies. The Human Microbiome Project Consortium has established a population-scale framework to study a variety of microbial communities that exist throughout the human body, enabling the generation of a range of quality-controlled data as well as community resources. The Human Microbiome Project (HMP), supported by the National Institutes of Health Common Fund, has the goal of characterizing the microbial communities that inhabit and interact with the human body in sickness and in health. In two Articles in this issue of Nature, the HMP Consortium presents the first population-scale details of the organismal and functional composition of the microbiota across five areas of the body. An associated News & Views discusses the initial results — which, along with those of a series of co-publications, already constitute the most extensive catalogue of organisms and genes related to the human microbiome yet published — and highlights some of the major questions that the project will tackle in the next few years.
Key Findings
1
Resources were generated from 242 healthy adults sampled at 15 or 18 body sites up to three times, producing 5,177 16S rRNA microbial taxonomic profiles.
2
The Human Microbiome Project Consortium established a population-scale framework with standardized metagenomic protocols for human microbiome research.
3
The project produced over 3.5 terabases of metagenomic sequence data and approximately 800 sequenced reference strains isolated from the human body.
4
These combined datasets constitute the largest resource describing human microbiome abundance and variety and provide a framework for future studies.
Research Object
Human microbiome (microbial communities and genes across multiple human body sites)
Research Subject
Population-scale framework and resources for characterizing abundance, diversity, and metagenomic profiles of the human microbiome across multiple body sites
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2012-06-01
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