Structure, function and diversity of the healthy human microbiome

Структура, функции и разнообразие микробиома здорового человека
Catherine Lozupone, Rob Knight, Jason Miller, Sergey Koren, Mathangi Thiagarajan, Sarah Young, Qiandong Zeng, Jennifer R. Wortman, Bruce W. Birren, William Nelson, Todd Z. DeSantis, Gary L. Andersen, Scott T. Kelley, Dan Knights, Ruth E. Ley, Daniel McDonald, R. C. Edgar, Victor Markowitz, Konstantinos Mavromatis, I-Min A. Chen, Ken Chu, Nikos C. Kyrpides, Brian J. Haas, José C. Clemente, Katherine S. Pollard, Barbara A. Methé, Konstantinos Liolios, Krishna Palaniappan, Patrick D. Schloss, Curtis Huttenhower, Nicola Segata, Janet Jansson, Jacques Izard, Dirk Gevers, Eric J. Alm, Antonio González, Todd J. Treangen, Martin J. Blaser, Sahar Abubucker, Jonathan H. Badger, Chien‐Chi Lo, Patrick Chain, Bo Liu, Richard A. Gibbs, Richard K. Wilson, Lucinda Fulton, Robert S. Fulton, Elaine R. Mardis, Yuanqing Wu, Lei Chen, Carsten Russ, Kristine M. Wylie, Jack D. Sobel, George M. Weinstock, Erica Sodergren, Georgia Giannoukos, Chad Nusbaum, Christie Kovar, Donna M. Muzny, Ramana Madupu, Heidi H. Kong, Jeffery A. Schloss, Peter Mannon, Jonathan Crabtree, Owen White, Joshua Orvis, Jamison McCorrison, Indresh Singh, Asif Chinwalla, Patrick Minx, Sandra W. Clifton, Donna M. Muzny, Kim C. Worley, Kris A. Wetterstrand, Jeroen Raes, Thomas M. Schmidt, Kevin Riehle, Mihai Pop, Wesley C. Warren, Sarah Young, John C. Martin, Omry Koren, Floyd E. Dewhirst, Heather H. Creasy, Ashlee M. Earl, Michael G. FitzGerald, Michelle Giglio, Kymberlie Hallsworth-Pepin, Elizabeth A. Lobos, Vincent Magrini, John Martin, Makedonka Mitreva, James Versalovic, Aye Wollam, Kjersti M. Aagaard, Olukemi O. Abolude, Emma Allen‐Vercoe, Lucia Alvarado, Scott Anderson, Elizabeth L. Appelbaum, Harindra Arachchi, Gary C. Armitage, Cesar Arze, Tulin Ayvaz, Carl C. Baker, Lisa Begg, Tsegahiwot Belachew, Veena Bhonagiri, Monika Bihan, Toby Bloom, Vivien Bonazzi, J. Paul Brooks, Gregory A. Buck, Christian Buhay, Dana Busam, Joseph L. Campbell, Shane R. Canon, Brandi L. Cantarel, I.-Min A. Chen, Shaila Chhibba, Dawn Ciulla, Sean Conlan, Jonathan Crabtree, Mary A. Cutting, Noam J. Davidovics, Catherine Davis, Carolyn Deal, Kimberley D. Delehaunty, Elena Deych, Yan Ding, David J. Dooling, Shannon Dugan, W. Michael Dunne, A. Scott Durkin, Rachel Erlich, Candace N. Farmer, Ruth M. Farrell, Karoline Faust, Michael Feldgarden, Victor Felix, Sheila Fisher, Anthony A. Fodor, Larry J. Forney, Leslie Foster, Valentina Di Francesco, Jonathan Friedman, Dennis C. Friedrich, Catrina C. Fronick, Hongyu Gao, M. Nathalia Garcia, Christina Giblin, Maria Y. Giovanni, Jonathan M. Goldberg, Johannes B. Goll, Antonio González, Allison Griggs, Sharvari Gujja, Susan Kinder Haake, Holli Hamilton, Emily Harris, Theresa A. Hepburn, Brandi Herter, Diane E. Hoffmann, Michael Holder, Clinton Howarth, Katherine Huang, Susan M. Huse, Huaiyang Jiang, Catherine Jordan, Vandita Joshi, James A. Katancik, Wendy A. Keitel, Cristyn Kells, Nicholas B. King, Karthik Kota, Patricio S. La Rosa, Sandra L. Lee, Katherine P. Lemon, Niall J. Lennon, Cecil M. Lewis, Lora Lewis, Kelvin Li, Bo Liu, Yue Liu, Chien- Chi Lo, R. Dwayne Lunsford, Tessa Madden, Anup Mahurkar, Daniel McDonald, Jean E. McEwen, Amy L. McGuire, Pamela McInnes, Teena Mehta, Kathie A. Mihindukulasuriya, Irene Newsham, Michelle Oglaughlin, Ioanna Pagani, Shital M. Patel, M.A. Pearson, Jane Peterson, Mircea Podar, Craig Pohl, Margaret Priest, Lita M. Proctor, Xiang Qin, Jacques Ravel, Jeffrey G. Reid, Mina Rho, Rosamond Rhodes, Maria C. Rivera, Beltran Rodriguez-Mueller, Yu Hui Rogers, Matthew C. Ross, Ravi Sanka, Pamela Sankar, J. Fah Sathirapongsasuti, Matthew Scholz, Lynn M. Schriml, Alyxandria M. Schubert, Julia A. Segre, William D. Shannon, Richard R. Sharp, Thomas J. Sharpton, Narmada Shenoy, Nihar U. Sheth, Gina Simone, Christopher S. Smillie, Doron D. Sommer, Paul Spicer, Granger G. Sutton, Sean M. Sykes, Diana Tabbaa, Chad Tomlinson, Manolito Torralba, Rebecca Truty, Tatiana A. Vishnivetskaya, Jason Walker, Lu Wang, Zhengyuan Wang, Doyle V. Ward, Mark A. Watson, Matthew T. Wheeler, James R. White, Katarzyna Wilczek-Boney, Todd Wylie, Chandri Yandava, Ye Liang, Yuzhen Ye, Shibu Yooseph, Bonnie P. Youmans, Lan Zhang, Yanjiao Zhou, Yiming Zhu, Laurie Zoloth, Jeremy Zucker, Susan J. Birren, Richard A. Gibbs, Sarah K. Highlander, Joseph F. Petrosino, Mircea Podar, Thomas J. Sharpton, Rebecca M. Truty, Katherine S. Pollard, Mihai Pop, Mina Rho, Yuzhen Ye, Rosamond Rhodes, Kevin P. Riehle, Pamela Sankar, Alyxandria M. Schubert, Patrick D. Schloss, Thomas M. Schmidt, Gina A. Simone, Jack D. Sobel, Todd J. Treangen, Shibu Yooseph, Laurie Zoloth, Joseph F. Petrosino, Julia A. Segre, Sean Conlan, Owen White, Lora Lewis, Yue Liu, Michelle D. O’Laughlin, Anthony A. Fodor, Ioanna Pagani, Liang Ye, Curtis Huttenhower, Jonathan Friedman, Michelle O’Laughlin, Yu-Hui Rogers, Sarah K. Highlander, James Versalovic, Joseph L. Campbell, Christopher Wellington, Gregory A. Buck, Maria C. Rivera, Chien-Chi Lo, I-Min A. Chen, Karoline Faust, Jeroen Raes, Yu-Hui Rogers, Zhengyuan Wang, Lei Chen, A. Scott Durkin, Jane A. Peterson, Xiang Qin, Yuanqing Wu, Karen E. Nelson, Nathalia Garcia, Daniel D. Sommer, Leslie Foster, Katherine P. Lemon, Jane Peterson, Dirk Gevers, Michael Feldgarden, Brandi Herter, A. Scott Durkin, Manolito Torralba, Irene Newsham, Gary L. Andersen, Gary Armitage, Carolyn Deal, Valentina Di Francesco, Shaila Chhibba, Nihar U. Sheth, Matthew Scholz, Victor M. Markowitz, Konstantinos Mavromatis, Ken Chu, Mary A. Cutting, Nicholas B. King, Heidi H. Kong, Omry Koren, Ruth E. Ley, Cecil M. Lewis, Bruce W. Birren, Brandi Herter, Michelle O'Laughlin, M A Torralba, Karen Nelson
2012-06-01

Body habitat niche specializationHuman Microbiome ProjectHuman microbiomeMetagenomic metabolic pathwaysMicrobial community structure
Studies of the human microbiome have revealed that even healthy individuals differ remarkably in the microbes that occupy habitats such as the gut, skin and vagina. Much of this diversity remains unexplained, although diet, environment, host genetics and early microbial exposure have all been implicated. Accordingly, to characterize the ecology of human-associated microbial communities, the Human Microbiome Project has analysed the largest cohort and set of distinct, clinically relevant body habitats so far. We found the diversity and abundance of each habitat’s signature microbes to vary widely even among healthy subjects, with strong niche specialization both within and among individuals. The project encountered an estimated 81–99% of the genera, enzyme families and community configurations occupied by the healthy Western microbiome. Metagenomic carriage of metabolic pathways was stable among individuals despite variation in community structure, and ethnic/racial background proved to be one of the strongest associations of both pathways and microbes with clinical metadata. These results thus delineate the range of structural and functional configurations normal in the microbial communities of a healthy population, enabling future characterization of the epidemiology, ecology and translational applications of the human microbiome. The Human Microbiome Project Consortium reports the first results of their analysis of microbial communities from distinct, clinically relevant body habitats in a human cohort; the insights into the microbial communities of a healthy population lay foundations for future exploration of the epidemiology, ecology and translational applications of the human microbiome. The Human Microbiome Project (HMP), supported by the National Institutes of Health Common Fund, has the goal of characterizing the microbial communities that inhabit and interact with the human body in sickness and in health. In two Articles in this issue of Nature, the HMP Consortium presents the first population-scale details of the organismal and functional composition of the microbiota across five areas of the body. An associated News & Views discusses the initial results — which, along with those of a series of co-publications, already constitute the most extensive catalogue of organisms and genes related to the human microbiome yet published — and highlights some of the major questions that the project will tackle in the next few years.
1
Ethnic or racial background was among the strongest associations linking microbial pathways and taxa to clinical metadata.
2
Healthy individuals showed wide variation in the diversity and abundance of signature microbes across gut, skin, vaginal, and other body habitats.
3
Metagenomic metabolic pathway carriage remained relatively stable across individuals despite substantial differences in community structure.
4
Microbial communities exhibited strong niche specialization both within individuals and between different people.
5
The Human Microbiome Project sampled an estimated 81–99% of genera, enzyme families, and community configurations found in the healthy Western microbiome.
6
The study establishes reference ranges of structural and functional configurations for healthy human microbial communities.

Healthy human microbiome (microbial communities occupying body habitats such as gut, skin, and vagina)

The structural and functional diversity, community configurations, niche specialization, and metabolic-pathway stability of the healthy human microbiome

Publication Details
Publication Date
2012-06-01
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Authors
Catherine Lozupone
Rob Knight
Jason Miller
Sergey Koren
Mathangi Thiagarajan
Sarah Young
Qiandong Zeng
Jennifer R. Wortman
Bruce W. Birren
William Nelson
Todd Z. DeSantis
Gary L. Andersen
Scott T. Kelley
Dan Knights
Ruth E. Ley
Daniel McDonald
R. C. Edgar
Victor Markowitz
Konstantinos Mavromatis
I-Min A. Chen
Ken Chu
Nikos C. Kyrpides
Brian J. Haas
José C. Clemente
Katherine S. Pollard
Barbara A. Methé
Konstantinos Liolios
Krishna Palaniappan
Patrick D. Schloss
Curtis Huttenhower
Nicola Segata
Janet Jansson
Jacques Izard
Dirk Gevers
Eric J. Alm
Antonio González
Todd J. Treangen
Martin J. Blaser
Sahar Abubucker
Jonathan H. Badger
Chien‐Chi Lo
Patrick Chain
Bo Liu
Richard A. Gibbs
Richard K. Wilson
Lucinda Fulton
Robert S. Fulton
Elaine R. Mardis
Yuanqing Wu
Lei Chen
Carsten Russ
Kristine M. Wylie
Jack D. Sobel
George M. Weinstock
Erica Sodergren
Georgia Giannoukos
Chad Nusbaum
Christie Kovar
Donna M. Muzny
Ramana Madupu
Heidi H. Kong
Jeffery A. Schloss
Peter Mannon
Jonathan Crabtree
Owen White
Joshua Orvis
Jamison McCorrison
Indresh Singh
Asif Chinwalla
Patrick Minx
Sandra W. Clifton
Donna M. Muzny
Kim C. Worley
Kris A. Wetterstrand
Jeroen Raes
Thomas M. Schmidt
Kevin Riehle
Mihai Pop
Wesley C. Warren
Sarah Young
John C. Martin
Omry Koren
Floyd E. Dewhirst
Heather H. Creasy
Ashlee M. Earl
Michael G. FitzGerald
Michelle Giglio
Kymberlie Hallsworth-Pepin
Elizabeth A. Lobos
Vincent Magrini
John Martin
Makedonka Mitreva
James Versalovic
Aye Wollam
Kjersti M. Aagaard
Olukemi O. Abolude
Emma Allen‐Vercoe
Lucia Alvarado
Scott Anderson
Elizabeth L. Appelbaum
Harindra Arachchi
Gary C. Armitage
Cesar Arze
Tulin Ayvaz
Carl C. Baker
Lisa Begg
Tsegahiwot Belachew
Veena Bhonagiri
Monika Bihan
Toby Bloom
Vivien Bonazzi
J. Paul Brooks
Gregory A. Buck
Christian Buhay
Dana Busam
Joseph L. Campbell
Shane R. Canon
Brandi L. Cantarel
I.-Min A. Chen
Shaila Chhibba
Dawn Ciulla
Sean Conlan
Jonathan Crabtree
Mary A. Cutting
Noam J. Davidovics
Catherine Davis
Carolyn Deal
Kimberley D. Delehaunty
Elena Deych
Yan Ding
David J. Dooling
Shannon Dugan
W. Michael Dunne
A. Scott Durkin
Rachel Erlich
Candace N. Farmer
Ruth M. Farrell
Karoline Faust
Michael Feldgarden
Victor Felix
Sheila Fisher
Anthony A. Fodor
Larry J. Forney
Leslie Foster
Valentina Di Francesco
Jonathan Friedman
Dennis C. Friedrich
Catrina C. Fronick
Hongyu Gao
M. Nathalia Garcia
Christina Giblin
Maria Y. Giovanni
Jonathan M. Goldberg
Johannes B. Goll
Antonio González
Allison Griggs
Sharvari Gujja
Susan Kinder Haake
Holli Hamilton
Emily Harris
Theresa A. Hepburn
Brandi Herter
Diane E. Hoffmann
Michael Holder
Clinton Howarth
Katherine Huang
Susan M. Huse
Huaiyang Jiang
Catherine Jordan
Vandita Joshi
James A. Katancik
Wendy A. Keitel
Cristyn Kells
Nicholas B. King
Karthik Kota
Patricio S. La Rosa
Sandra L. Lee
Katherine P. Lemon
Niall J. Lennon
Cecil M. Lewis
Lora Lewis
Kelvin Li
Bo Liu
Yue Liu
Chien- Chi Lo
R. Dwayne Lunsford
Tessa Madden
Anup Mahurkar
Daniel McDonald
Jean E. McEwen
Amy L. McGuire
Pamela McInnes
Teena Mehta
Kathie A. Mihindukulasuriya
Irene Newsham
Michelle Oglaughlin
Ioanna Pagani
Shital M. Patel
M.A. Pearson
Jane Peterson
Mircea Podar
Craig Pohl
Margaret Priest
Lita M. Proctor
Xiang Qin
Jacques Ravel
Jeffrey G. Reid
Mina Rho
Rosamond Rhodes
Maria C. Rivera
Beltran Rodriguez-Mueller
Yu Hui Rogers
Matthew C. Ross
Ravi Sanka
Pamela Sankar
J. Fah Sathirapongsasuti
Matthew Scholz
Lynn M. Schriml
Alyxandria M. Schubert
Julia A. Segre
William D. Shannon
Richard R. Sharp
Thomas J. Sharpton
Narmada Shenoy
Nihar U. Sheth
Gina Simone
Christopher S. Smillie
Doron D. Sommer
Paul Spicer
Granger G. Sutton
Sean M. Sykes
Diana Tabbaa
Chad Tomlinson
Manolito Torralba
Rebecca Truty
Tatiana A. Vishnivetskaya
Jason Walker
Lu Wang
Zhengyuan Wang
Doyle V. Ward
Mark A. Watson
Matthew T. Wheeler
James R. White
Katarzyna Wilczek-Boney
Todd Wylie
Chandri Yandava
Ye Liang
Yuzhen Ye
Shibu Yooseph
Bonnie P. Youmans
Lan Zhang
Yanjiao Zhou
Yiming Zhu
Laurie Zoloth
Jeremy Zucker
Susan J. Birren
Richard A. Gibbs
Sarah K. Highlander
Joseph F. Petrosino
Mircea Podar
Thomas J. Sharpton
Rebecca M. Truty
Katherine S. Pollard
Mihai Pop
Mina Rho
Yuzhen Ye
Rosamond Rhodes
Kevin P. Riehle
Pamela Sankar
Alyxandria M. Schubert
Patrick D. Schloss
Thomas M. Schmidt
Gina A. Simone
Jack D. Sobel
Todd J. Treangen
Shibu Yooseph
Laurie Zoloth
Joseph F. Petrosino
Julia A. Segre
Sean Conlan
Owen White
Lora Lewis
Yue Liu
Michelle D. O’Laughlin
Anthony A. Fodor
Ioanna Pagani
Liang Ye
Curtis Huttenhower
Jonathan Friedman
Michelle O’Laughlin
Yu-Hui Rogers
Sarah K. Highlander
James Versalovic
Joseph L. Campbell
Christopher Wellington
Gregory A. Buck
Maria C. Rivera
Chien-Chi Lo
I-Min A. Chen
Karoline Faust
Jeroen Raes
Yu-Hui Rogers
Zhengyuan Wang
Lei Chen
A. Scott Durkin
Jane A. Peterson
Xiang Qin
Yuanqing Wu
Karen E. Nelson
Nathalia Garcia
Daniel D. Sommer
Leslie Foster
Katherine P. Lemon
Jane Peterson
Dirk Gevers
Michael Feldgarden
Brandi Herter
A. Scott Durkin
Manolito Torralba
Irene Newsham
Gary L. Andersen
Gary Armitage
Carolyn Deal
Valentina Di Francesco
Shaila Chhibba
Nihar U. Sheth
Matthew Scholz
Victor M. Markowitz
Konstantinos Mavromatis
Ken Chu
Mary A. Cutting
Nicholas B. King
Heidi H. Kong
Omry Koren
Ruth E. Ley
Cecil M. Lewis
Bruce W. Birren
Brandi Herter
Michelle O'Laughlin
M A Torralba
Karen Nelson
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