Determination of Catechol by a Novel Laccase Biosensor Based on Zinc-Oxide Sol-Gel
Определение катехола с помощью нового биосенсора на основе лакказы в сол‑геле оксида цинка
2015-02-18
SCID: 54.1/535wrgz7
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ZnO sol-gelalternating current impedancecatechol determinationchitosan/ZnO/glassy carbon electrodelaccase biosensor
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Abstract (AI)
A biosensor was fabricated by incorporating laccase in a ZnO sol-gel with chitosan as a matrix for the determination of catechol. The ZnO nanoparticles were characterized by X-ray diffraction and atomic force microscopy. The conductivity of the chitosan/ZnO/glassy carbon electrode film was investigated by alternating current impedance. The biosensor was employed to monitor the reduction of catechol, and the peak current increased linearly with concentration between 1.0 × 10−6 and 1.0 × 10−4 mole per liter with a limit of detection of 2.9 × 10−7 mole per liter. The laccase biosensor exhibited good stability, reproducibility, and some selectivity.
Key Findings
1
A novel laccase biosensor was fabricated by incorporating laccase into a ZnO sol-gel with chitosan as the matrix for catechol determination
2
Conductivity of the chitosan/ZnO/glassy carbon electrode film was investigated by alternating current impedance
3
The biosensor limit of detection for catechol was 2.9 × 10−7 M
4
The biosensor monitored catechol reduction with a linear peak current response from 1.0 × 10−6 to 1.0 × 10−4 M
5
The laccase biosensor demonstrated good stability, reproducibility, and some selectivity
6
ZnO nanoparticles in the sol-gel were characterized using X-ray diffraction and atomic force microscopy
Research Object
Laccase-based ZnO sol–gel/chitosan biosensor on a glassy carbon electrode for catechol determination
Research Subject
Analytical performance for catechol detection including linear range, limit of detection, peak-current response, stability, reproducibility, and selectivity of the biosensor
Publication Details
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2015-02-18
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